Project

Part:BBa_K404112:Design

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)

Cytosine deaminase (CD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cytosine deaminase was PCR amplified using the codA gene as PCR template. Additionally, two site-directed mutagenesis had to be performed in order to delete PsI and NgoMIV.


Source

codA

References